Effect of an ald gene knock-out on spore revival

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Video in TIB AV-Portal: Effect of an ald gene knock-out on spore revival

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Title
Effect of an ald gene knock-out on spore revival
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CC Attribution 3.0 Germany:
You are free to use, adapt and copy, distribute and transmit the work or content in adapted or unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor.
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Release Date
2017
Language
Silent film

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Abstract
Down- and up-shift responses of a mutant colony of strain BIB1416 (Δald , PtrpE-mCherry) and a non-fluorescent WT colony BIB224. Both strains were starved in co-culture on an SM* gel pad and show comparable sporulation dynamics. After sporulation is complete, spore revival was induced with L-alanine. Both mutant and WT spores show comparable germination. However, only WT spores grew out while the mutant spores did not.
Keywords phenotypic heterogeneity heterochronicity starvation sporulation germination outgrowth
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