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Effect of an ald gene knock-out on spore revival

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Title Effect of an ald gene knock-out on spore revival
Title of Series Supplemental Videos of “Phenotypic memory in Bacillus subtilis links dormancy entry and exit by a spore quantity-quality tradeoff”
Author Mutlu, Alper
Trauth, Stephanie
Ziesack, Marika
Nagler, Katja
Bergeest, Jan-Philip
Rohr, Karl
Becker, Nils
Höfer, Thomas
Bischofs, Ilka B.
License CC Attribution 3.0 Germany:
You are free to use, adapt and copy, distribute and transmit the work or content in adapted or unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor.
DOI 10.5446/34001
Publisher BioQuant Center of the University of Heidelberg (BioQuant), Max-Planck-Institute for Terrestrial Microbiology
Release Date 2017
Language Silent film

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Subject Area Life Sciences
Abstract Down- and up-shift responses of a mutant colony of strain BIB1416 (Δald , PtrpE-mCherry) and a non-fluorescent WT colony BIB224. Both strains were starved in co-culture on an SM* gel pad and show comparable sporulation dynamics. After sporulation is complete, spore revival was induced with L-alanine. Both mutant and WT spores show comparable germination. However, only WT spores grew out while the mutant spores did not.
Keywords phenotypic heterogeneity
heterochronicity
starvation
sporulation
germination
outgrowth

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