Effect of accelerating sporulation via overexpression of KinA

Video in TIB AV-Portal: Effect of accelerating sporulation via overexpression of KinA

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Title
Effect of accelerating sporulation via overexpression of KinA
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CC Attribution 3.0 Germany:
You are free to use, adapt and copy, distribute and transmit the work or content in adapted or unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor.
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Release Date
2017
Language
Silent film

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Abstract
Down- and up-shift responses of a mutant colony of strain BIB1332 (Pspank-kinA) and a WT colony BIB1126 (PrapA-mCherry). Both strains were starved in co-culture on a SM* gel pad, which was supplemented with IPTG to induce KinA in the mutant strain and accelerate its sporulation. After sporulation was complete, spore revival was induced with L-alanine.
Keywords Phenotypic memory phenotypic heterogeneity heterochronicity starvation sporulation germination outgrowth phosphorelay rapA kinA pmCherry-Star quality versus quantity tradeoff network complexity complex phenotypes carry-over
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