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Effect of Ald re-programming on B. subtilis spore revival

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Title Effect of Ald re-programming on B. subtilis spore revival
Title of Series Supplemental Videos of “Phenotypic memory in Bacillus subtilis links dormancy entry and exit by a spore quantity-quality tradeoff”
Author Mutlu, Alper
Trauth, Stephanie
Ziesack, Marika
Nagler, Katja
Bergeest, Jan-Philip
Rohr, Karl
Becker, Nils
Höfer, Thomas
Bischofs, Ilka B.
License CC Attribution 3.0 Germany:
You are free to use, adapt and copy, distribute and transmit the work or content in adapted or unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor.
DOI 10.5446/33996
Publisher BioQuant Center of the University of Heidelberg (BioQuant), Max-Planck-Institute for Terrestrial Microbiology
Release Date 2017
Language Silent film

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Subject Area Life Sciences
Abstract Down- and up-shift responses of strain BIB1300, which harbors a PrapA-mCherry promoter fusion that reports on sporulation timing and in which Ald expression can be controlled by an IPTG-inducible promoter. Cells were spotted onto an SM* gel pad. At the indicated time IPTG was added to the pad. This results in the expression of Ald in the progenitor cells of late spores. After sporulation was complete, spore revival was induced with L-alanine. The spores that will grow out are circled. Note that red-fluorescent late spores are able to grow out successfully.
Keywords phenotypic heterogeneity
heterochronicity
starvation
sporulation
germination
outgrowth
phosphorelay
rapA
pmCherry-Star
Phenotypic memory
carry-over
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AV-Portal 3.8.0 (dec2fe8b0ce2e718d55d6f23ab68f0b2424a1f3f)