Effect of Ald re-programming on B. subtilis spore revival

Video in TIB AV-Portal: Effect of Ald re-programming on B. subtilis spore revival

Formal Metadata

Title
Effect of Ald re-programming on B. subtilis spore revival
Title of Series
Author
Mutlu, Alper
Trauth, Stephanie
Ziesack, Marika
Nagler, Katja
Bergeest, Jan-Philip
Rohr, Karl
Becker, Nils
Höfer, Thomas
Bischofs, Ilka B.
License
CC Attribution 3.0 Germany:
You are free to use, adapt and copy, distribute and transmit the work or content in adapted or unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor.
Identifiers
Publisher
Release Date
2017
Language
Silent film

Content Metadata

Subject Area
Abstract
Down- and up-shift responses of strain BIB1300, which harbors a PrapA-mCherry promoter fusion that reports on sporulation timing and in which Ald expression can be controlled by an IPTG-inducible promoter. Cells were spotted onto an SM* gel pad. At the indicated time IPTG was added to the pad. This results in the expression of Ald in the progenitor cells of late spores. After sporulation was complete, spore revival was induced with L-alanine. The spores that will grow out are circled. Note that red-fluorescent late spores are able to grow out successfully.
Keywords phenotypic heterogeneity heterochronicity starvation sporulation germination outgrowth phosphorelay rapA pmCherry-Star Phenotypic memory carry-over
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Timings

  256 ms - page object

Version

AV-Portal 3.20.1 (bea96f1033d39fbe77f82542458e108105398441)
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