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The down- and up-shift responses on an SM* gel pad of the fluorescent marker strain BIB1126, which carries a PrapA-mCherry promoter fusion that reports on sporulation timing.

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Title The down- and up-shift responses on an SM* gel pad of the fluorescent marker strain BIB1126, which carries a PrapA-mCherry promoter fusion that reports on sporulation timing.
Title of Series Supplemental Videos of “Phenotypic memory in Bacillus subtilis links dormancy entry and exit by a spore quantity-quality tradeoff”
Author Mutlu, Alper
Trauth, Stephanie
Ziesack, Marika
Nagler, Katja
Bergeest, Jan-Philip
Rohr, Karl
Becker, Nils
Höfer, Thomas
Bischofs, Ilka B.
License CC Attribution 3.0 Germany:
You are free to use, adapt and copy, distribute and transmit the work or content in adapted or unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor.
DOI 10.5446/33994
Publisher BioQuant Center of the University of Heidelberg (BioQuant), Max-Planck-Institute for Terrestrial Microbiology
Release Date 2017
Language Silent film

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Subject Area Life Sciences
Abstract A few cells that initiated sporulation early were tracked with a yellow arrow, while some representative cells that delayed sporulation were followed with a green arrow. The latter strongly induce mCherry expression. After sporulation is complete, the marker nicely distinguishes between early and late spores. Spore revival was then induced by the addition of L-alanine. All spores that grew out in response to induction with L-alanine are circled. They all show low fluorescence.
Keywords phenotypic heterogeneity
heterochronicity
starvation
sporulation
germination
outgrowth
phosphorelay
rapA
pmCherry-Star

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