A few cells that initiated sporulation early were tracked with a yellow arrow, while some representative cells that delayed sporulation were followed with a green arrow. The latter strongly induce mCherry expression. After sporulation is complete, the marker nicely distinguishes between early and late spores. Spore revival was then induced by the addition of L-alanine. All spores that grew out in response to induction with L-alanine are circled. They all show low fluorescence.