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C-terminal mCherry tagged Ald expression from its native promoter

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Title C-terminal mCherry tagged Ald expression from its native promoter
Title of Series Supplemental Videos of “Phenotypic memory in Bacillus subtilis links dormancy entry and exit by a spore quantity-quality tradeoff”
Author Mutlu, Alper
Trauth, Stephanie
Ziesack, Marika
Nagler, Katja
Bergeest, Jan-Philip
Rohr, Karl
Becker, Nils
Höfer, Thomas
Bischofs, Ilka B.
License CC Attribution 3.0 Germany:
You are free to use, adapt and copy, distribute and transmit the work or content in adapted or unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor.
DOI 10.5446/33997
Publisher Max-Planck-Institute for Terrestrial Microbiology, BioQuant Center of the University of Heidelberg (BioQuant)
Release Date 2017
Language Silent film

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Subject Area Life Sciences
Abstract The down- and up-shift responses of strain BIB1423, which expresses fluorescently tagged Ald-mCherry from its native promoter (Pald-ald-mCherry). At the onset of sporulation, cells that sporulate early have higher fluorescence levels than cells that delay sporulation due to dilution. The fluorescence is carried from the progenitors into the developing spore. Upon spore release, fluorescence drops due to unknown reasons and then remains stable. Early spores show higher fluorescence than late spores. All spores that grew out in response to L-alanine stimulation were circled. They all show higher levels of fluorescence compared to non-outgrowing spores.
Keywords Phenotypic memory
phenotypic heterogeneity
heterochronicity
starvation
sporulation
germination
outgrowth
carry-over
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AV-Portal 3.8.2 (0bb840d79881f4e1b2f2d6f66c37060441d4bb2e)