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Heterochronic sporulation response of B. subtilis on an SM* gel pad and the subsequent spore revival upon addition of L-alanine.

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Logo of Max-Planck-Institute for Terrestrial MicrobiologyMax-Planck-Institute for Terrestrial Microbiology
Logo of BioQuant Center of the University of Heidelberg (BioQuant)BioQuant Center of the University of Heidelberg (BioQuant)
 Mutlu, Alper Trauth, Stephanie Ziesack, Marika Nagler, Katja Bergeest, Jan-Philip Rohr, Karl Becker, Nils Höfer, Thomas Bischofs, Ilka B.

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Heterochronic sporulation response of B. subtilis on an SM* gel pad and the subsequent spore revival upon addition of L-alanine.
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CC Attribution 3.0 Germany:
You are free to use, adapt and copy, distribute and transmit the work or content in adapted or unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor.
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Abstract
The strain BIB1019 carries PtrpE*-mCherry and PspoIIE-gfp reporters. After 90 h of starvation, spores have been released from the mother cell. To visualize the distribution of early and late spores prior to the nutrient up-shift, false colored frames were included with early and late spores denoted in yellow and green, respectively. Note that most spores geminated but only early spores succeed in resuming vegetative growth. Spores that grew out upon nutrient stimulation are circled in red.
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