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Effect of KinA induction on sporulation and spore revival of the Pald Ald-mCherry reporter strain

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Title Effect of KinA induction on sporulation and spore revival of the Pald Ald-mCherry reporter strain
Title of Series Supplemental Videos of “Phenotypic memory in Bacillus subtilis links dormancy entry and exit by a spore quantity-quality tradeoff”
Author Mutlu, Alper
Trauth, Stephanie
Ziesack, Marika
Nagler, Katja
Bergeest, Jan-Philip
Rohr, Karl
Becker, Nils
Höfer, Thomas
Bischofs, Ilka B.
License CC Attribution 3.0 Germany:
You are free to use, adapt and copy, distribute and transmit the work or content in adapted or unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor.
DOI 10.5446/34000
Publisher BioQuant Center of the University of Heidelberg (BioQuant), Max-Planck-Institute for Terrestrial Microbiology
Release Date 2017
Language Silent film

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Subject Area Life Sciences
Abstract Left: KinA was induced at t=0 resulting in accelerated sporulation. All KinA-induced spores are highly fluorescent and most are capable of outgrowth in response to L-alanine. Right: The induction of KinA was delayed (t=20h) and induced in the progenitor cells of late spores. While the highly fluorescent early spores grew out none of the resulting lowly fluorescent, late but KinA-induced spores grew out. This shows that KinA induction does not alter the spore properties per se.
Keywords Phenotypic memory
phenotypic heterogeneity
heterochronicity
starvation
sporulation
germination
outgrowth
phosphorelay
kinA
quality versus quantity tradeoff
network complexity
complex phenotypes
carry-over
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AV-Portal 3.8.0 (dec2fe8b0ce2e718d55d6f23ab68f0b2424a1f3f)