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Up-shift response of spores of the fluorescent marker strain PrapA-mCherry upon exposure to L-alanine.

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Title Up-shift response of spores of the fluorescent marker strain PrapA-mCherry upon exposure to L-alanine.
Title of Series Supplemental Videos of “Phenotypic memory in Bacillus subtilis links dormancy entry and exit by a spore quantity-quality tradeoff”
Author Mutlu, Alper
Trauth, Stephanie
Ziesack, Marika
Nagler, Katja
Bergeest, Jan-Philip
Rohr, Karl
Becker, Nils
Höfer, Thomas
Bischofs, Ilka B.
License CC Attribution 3.0 Germany:
You are free to use, adapt and copy, distribute and transmit the work or content in adapted or unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor.
DOI 10.5446/33995
Publisher BioQuant Center of the University of Heidelberg (BioQuant), Max-Planck-Institute for Terrestrial Microbiology
Release Date 2017
Language Silent film

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Subject Area Life Sciences
Abstract Spores were generated by the Sterlini-Mandelstam protocol in liquid shake-flask culture, and show a broadly heterogenous distribution of fluorescence. Spores were then spotted onto an SM gel pad and spore revival was induced by adding L-alanine.
Keywords phenotypic heterogeneity
heterochronicity
starvation
sporulation
germination
outgrowth
phosphorelay
rapA
pmCherry-Star
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AV-Portal 3.8.0 (dec2fe8b0ce2e718d55d6f23ab68f0b2424a1f3f)