Chromatography - 2. Analytical Techniques

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Title
Chromatography - 2. Analytical Techniques
Alternative Title
Chromatographie - 2. Analysentechniken
Author
License
CC Attribution - NonCommercial - NoDerivatives 3.0 Germany:
You are free to use, copy, distribute and transmit the work or content in unchanged form for any legal and non-commercial purpose as long as the work is attributed to the author in the manner specified by the author or licensor.
Identifiers
IWF Signature
C 1568
Publisher
Release Date
1985
Language
English
Producer
IWF
Production Year
1984

Technical Metadata

IWF Technical Data
Film, 16 mm, LT, 173 m ; F, 16 min

Content Metadata

Subject Area
Abstract
The equipment used in the techniques for making liquid and gaseous chromatographic separations as thin-layer chromatography, high-capacity column chromatography and gas chromatography are presented here. Deposit of samples, encouragement of the mobile phase and the qualitative as well as the quantitave evaluation of chromatograms all require different kinds of instrumentation, depending on the technique of chromatographic separation involved. Together, these different instruments form an independent system of analysis. The various combinations of appliances used in these techniques of chromatographic analysis are shown.
Keywords automatic analyser capillary column chromatography column chromatography development chamber gas chromatography immersion chamber photometer reaction detector separation column spray development / agent thin layer chromatography
Sorption Computer animation Chromatography Separator (milk) Chromophor Process (computing)
Anomalie <Medizin> Reaction mechanism Chromatography Elution Experiment indoor Separator (milk) Nanoparticle
High-performance liquid chromatography Thin film Elution Flux (metallurgy) Nanoparticle Separator (milk) Ion transporter
Digoxigenin Resin Chromatography Materials science Glasses Aluminium Ice sheet Ionenaustausch Separator (milk)
Chromatography Glasses Aage Mixture
Separator (milk)
Sample (material) Quantitative analysis (chemistry) Man page
Sample (material) Area
Sample (material) Ice sheet
Sample (material) Golgi apparatus Solvent Chromatography Reaktionsgleichung Gas Elution Substrat <Chemie> Separator (milk) Combustion chamber
Chromatin Chromatography Elution Separator (milk) Combustion chamber
Systemic therapy Chromatography Controller (control theory) Biscuit Elution Separator (milk) Combustion chamber
Sample (material) Separation process Gesundheitsstörung Meeting/Interview Chromatography Growth medium Elution Flux (metallurgy) Library (computing) Separator (milk) Ice front
Concentrate Chemical reaction Solution
Sample (material) High-performance liquid chromatography Computer animation Thin film Ion transporter Block (periodic table)
Coiled coil Separation process Solution Pressure Food additive Sample (material) Systemic therapy Volumetric flow rate High-performance liquid chromatography Dünnschichtchromatographie Elution Experiment indoor Flux (metallurgy) Mixing (process engineering) Butcher
Sprayer Breed standard Separator (milk) Chemical reaction
Glasses Colourant Tube (container) Flux (metallurgy) Mixing (process engineering) Chemical reaction Solution
Spectral line Amber
Intergranular corrosion Fluorescence Ultraviolettspektrum Gas chromatography Experiment indoor Separator (milk) Chromophor
Flame Hydrogen Combustibility Lecture/Conference Iron Ocean current Substrat <Chemie> Separator (milk) Electronic cigarette
Sample (material) Separator (milk)
Computer animation Lecture/Conference Thermoforming Nitrosamine
we will cry with dog a 3 a 2 look analytical techniques chromatographic separation can be performed with the help of different techniques we 1st remember the traditional gas chromophore graph it uses and sorption and partition processes for gas phase separation liquid
chromatography in columns uses a variety of separation mechanisms with relatively big particles in the mobile phase a pedestal to pump is sufficient for propelling the liquid mobile phase we're very small
particles contained in thin columns precision
high-pressure pump is needed
however to affect the optimal flux of mobile phase very fast separations are performed with this so-called high-performance liquid chromatography all HPLC here showed in real time modem thin layer
chromatography will give comparable results with relatively simple equipment
much more time will be needed however for good separation the film lab chromatography yes In sheets of Aluminium class dig call glasses of different dimensions serve as support for various stationary phase materials such as for instance and sorbents Joe and ion-exchange resins in roughly 0 . 1 mm less for
qualitative results liquid mixture containing the substances to be separated constantly be applied to the stationary
phase with glass microcode emeritus old but different
Spanish proceeding of Tim lachrymatory graphic separation Gambia able automated to perform
quantitative analysis done too parts per billion dimensions modern analytical equivalent serves
for exact calibration of evil man Alito volumes before the sample is
collected the calibrated
Kabila areas 1st cleaned and trimmed or the damn good shows Moga was collected from the sample am poop difference
but also go samples are precisely blazed according to a program at Sodom distances on the starting line almost similar sheet the war
this apparatus automatically draw small lines on the substrate the sample is introduced by a small micros syringe if we will do it with Wolf film layer
chromatography uses different separation chambers in which the mobile phase Comey introduced in many ways this has a plane not bottom a relatively big volume is left for the gas phase result reproducibility of a separation essentially depend on the finally quit balance of mobile phase of gas as well as on the interaction of the gas-phase the evaporated solvents and the separating led but if In this so-called sandwich Chamber of things there is covered from both sides by a glass pane the absence of a gas phase which would otherwise fluence good drive there guarantees a good reproducibility of separation linear
development of chromatin grounds is shown in this chamber here only a relatively small volume of the mobile phase is needed the chromatographic separations Combie started from 2 0
opposite sides In this system a good control of the separation is possible this
so-called you chamber is used in Cole said killer chromatography the mobile phase is introduced by way of a say range within the circumference of the biscuit
many different samples committee position With that and circular development the mobile phase is introduced around the periphery of the days in this way Alachua division is prevented which normally would lead to enlarge spots of the substances the flux of the mobile phase committee kept constant during the separation process much better than in the other devices shown before due to the fact that With linear development of flux those dumb when the front proceeds into stationary phase secular development as share media use is essentially for the mobile phase it is obvious that especially news of a starting point the separation a shop of them with linear developed armed even ideal conditions the resolution committee improved if the sample is introduced in lines instead of dots at the starting position
chromatography is by no means restricted to colored substances here the separated colorless substances are sprayed on
and after development show colored reaction products on them much
faster and better results could be achieved by Amersham into the reagent solution with this automated lift thereafter concentrations or quantities can be measured with a automata this
special Finlay scanner Prince I Bozo Demetri goal sura Madrid results from evaluated thinned then Cromer digram with and position of the
single spots are transformed into a chromatographic plots comparable with those resulting
from columns an integrated calculates the prince the intensity of the single bands which later commit correlated with those received from GEC
and HPLC plots high-performance liquid chromatography HPLC this HPLC device consists of 1 block for the introduction of the a sample of a high-pressure pump thin column filled with
articles of a few microns in diameter of flux detector plot and the integrator with printer here we introduce a solution of mixed substances with us arranged by the septum of the entrance pulled into the sample coil high-performance liquid chromatography for short HPLC is likewise efficient chromatographic technique to be instrumental thin layer chromatography by
turning this valve be HPLC column is opened for the addition of the mobile phase this column system employs a special safety fitting for last cut to ship their stability them withstand high pressures after the war but here the separation process in real time these elution time needed to can be essentially reduced by gradient elution that is when continuously changed proportion moved to a different solvency is used as a mobile phase after the illusion that is when was separated substances have left the column then undetected by a flow for odometer
initially colorless substances have 1st to be transformed into colored ones by chemical reactions in the so-called reaction detector this command as well so the reaction with spray reagent somethin ladders following the standard
HPLC separation
reagent solutions I headed added directly at the outlet of a column to be Aleutian flux by tubes here Weikum watch the color reaction product passing a glass scored be introduced air bubbles cause a segmentation of the mobile phase and prevent the mixing of formally separated substances they
subsequently passed the
absorption so whether detected
the wavelength calibration 1st said the center of an absorption band of 1 of the substances and amber light
absorption is measure
ultraviolet absorption is detected with UV sensitive detector fluorescence with fluorescence detector and ions in the conductivity Seo gas chromatography GC in the founders of this gas chromophore graph a Accor separation column is heated at me it contains a
liquid-phase on solid substrate this compartment contains the detector at the end of a feminist or column respectively after combustion of the separated geisha substances in a
hydrogen a flame the resultant irons are detected as an ionization current by the flame ionization detector much has
separation efficiencies can be achieved in substrate freak a bidder is the liquid
sample is injected with a hypodermic needle into the sample in that septum passing before this it is evaporated at September just like a higher than that of a column against flags carries the separated substances through the column and to the ionization detector signals of the ionization
current form the league's over plot and integrated completed by providing quantitative
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