Patch Clamp Method
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| License | No Open Access License: German copyright law applies. This film may be used for your own use but it may not be distributed via the internet or passed on to external parties. | |
| Identifiers | 10.3203/IWF/C-12775eng (DOI) | |
| IWF Signature | C 12775 | |
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| Production Year | 2001 |
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| IWF Technical Data | Video-Clip ; F, 1 min 29 sec |
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Transcript: English(auto-generated)
00:05
The cell membrane serves as a barrier for most water-soluble molecules. Channels enable a regulated flow of ions through the membrane.
00:21
In the patch clamp method, a small membrane area which frequently contains only one or very few channels is selected. The contact between the microelectrode and the cell membrane is so tight that ions, and thus any electrical currents, can only flow through channels in the membrane.
00:44
In this manner, it's possible to measure current flowing through individual ion channels. The fluctuations show the opening and closing of the channels. Conclusions can be drawn as to the opening time and the strength of the ion fluxes.
01:06
If the membrane patch is separated from the cell, medium composition at both sides of the membrane can be controlled. Now it's possible to demonstrate that channels are selectively permeable for specific ions.