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Elektronenmikroskopische Präparationsmethoden - 3. Negativkontrastierung

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Formal Metadata

Title Elektronenmikroskopische Präparationsmethoden - 3. Negativkontrastierung
Alternative Title Electron Microscopic Preparation Techniques - 3. Negative Staining
Author Spiess, Eberhardt
Mayer, Frank
License No Open Access License:
German copyright law applies. This film may be used for your own use but it may not be distributed via the internet or passed on to external parties.
DOI 10.3203/IWF/C-1191
IWF Signature C 1191
Publisher IWF (Göttingen)
Release Date 1976
Language German
Producer IWF
Production Year 1975

Technical Metadata

IWF Technical Data Film, 16 mm, LT, 48 m ; F, 4 1/2 min

Content Metadata

Subject Area Life Sciences
Abstract Negativkontrastierung eines biologischen Objektes mit Uranylacetat. Abflottieren eines Kohleträgerfilms auf der Oberfläche einer Objektsuspension. An seiner Unterseite heften sich Objektpartikel an, die durch Überführen des Kohlefilms auf Wasser gewaschen und anschließend durch Flottieren des Films auf wäßriger Uranylacetatlösung negativkontrastiert werden.
Negative staining a biological specimen with uranyl acetate. Floating a carbon carrier film on specimen suspension. Specimen particles are adsorbed to its underside, washing the adsorbed particles by floating the film on water, negatively staining them by floating the carrier film on aqueous uranyl acetate solution.
Keywords Präparation / Elektronenmikroskop
Elektronenmikroskop / Präparationsmethoden
electron microscope / preparation methods
preparation / electron microscope

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