Vakzineherstellung zur Verhütung von Anaerobierinfektionen in den Tropen: Differenzierung von pathogenen Clostridien mit Hilfe der Dampfraum-Gaschromatographie. Anzüchtung der Bakterien im Fermenter, Toxingewinnung durch Kaskadenultrafiltration, Kontrolle der Toxinaktivität. Impfung.

A system is described which consists firstly of identifying pathogen anaerobes in tropical grazing lands by means of GLC and biometrical comparison of metabolically produced fatty acid patterns with such of known reference strains. Locality specific anaerobes may then be continuously cultivated in an especially designed bioreactor and their toxins separated through ultra-filtration. The filtration process is a continuous two step procedure. At first the microbes are separated from the spent broth with a hollow fiber of approx. 1 Mio. NMCO and in the second stage the toxin is concentrated and freed of allergenic peptides with a hollow fiber filter of 20.000 NMCO. The highly concentrated formalinized toxoid may be applicated intradermically beside the tail of the animal in a volume of 0,25 ml with a dermo-jet.