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01:11 Copernicus Publications Englisch 2018

FIB-SEM video of two strongly encrusted cable bacteria

FIB-SEM video composed of 501 electron backscatter images. After each image a section of 10 nm was removed and a new section was imaged. The video shows two filaments with a horizontal cross-sectional view along the length of the filament that can be seen on the right at the start of the video. The filament that is visible on the left at the start of the video follows a different direction and therefore the cross-section shows different angles. The thick layer of mineral encrustation appears as a dark seal around the filament, the ridge compartments and the cell content appear white. The encrustation is only found around the filament and not within the periplasmic space. Both filaments are strongly encrusted and the mineral crust of both filaments is partly cemented together which can be observed from 0:06 till 0:26. The encrustation is heterogeneous with an average thickness of ˜500 nm.
  • Erscheinungsjahr: 2018
  • Herausgeber: Copernicus Publications
  • Sprache: Englisch
01:15 Copernicus Publications Englisch 2018

FIB-SEM video of a weakly encrusted cable bacterium

FIB-SEM video composed of 525 electron backscatter images showing the horizontal cross-sectional view along the length of three cells of a weakly encrusted filament. After each image a section of 10 nm was removed with the ion beam before a new section was imaged. The mineral encrustation can be seen as a dark seal around the filament while the ridge compartments and the cell content are unstained and appear white, the shared periplasmic space is gray. The encrustation is only found around the filament and not within the periplasmic space. At time stamp 0:21 and 1:13 the cell-cell junctions can be observed. On average, ˜180 nm of encrustation surrounds the cable bacterium.
  • Erscheinungsjahr: 2018
  • Herausgeber: Copernicus Publications
  • Sprache: Englisch
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