C. elegans Behavioural Assay: Thermogradient
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Computer animation
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Bacteria
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Computer animation
Transcript: English(auto-generated)
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This protocol involves observing the behavior of worms in response to a temperature gradient, a process otherwise known as thermotaxis. The experiment is rather simple to set up as all you need is some C. elegans,
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which will have been prepared in advance for you, as well as an NGM plate, some M9 buffer, Eppendorf tubes, and a temperature gradient. The thermogradiant consists of an aluminium plate, which is immersed on either end in a 5°C or 35°C water bath. This induces a temperature gradient along the length of the aluminium,
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which creates zones of different temperatures that the worms will even migrate into or away from. Wet the plate with 1 milliliter of M9 buffer, making sure to evenly distribute around the plate. You then add the worms to a 1.5 milliliter vial and allow them to settle at the bottom. Remove 900 microliters of the supernatant and add the remaining 100 microliters of worms to your plate.
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Finally, you incubate the plate for one hour on a temperature gradient. At the end, you count the worms in each region, and your data can be used to calculate the TTX index.