C. elegans Behavioural Assay: Chemotaxis
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| Number of Parts | 11 | |
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| License | CC Attribution - NoDerivatives 4.0 International: You are free to use, copy, distribute and transmit the work or content in unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor. | |
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2
9
00:00
Computer animation
00:04
Bacteria
00:24
Bacteria
00:27
Bacteria
00:43
Bacteria
01:09
Computer animation
Transcript: English(auto-generated)
00:04
We'll study the worm's olfactory response to a chemical stimulus. Begin the procedure by dividing the plate into four equal quadrants by drawing the underside with a marker. At the center, draw a circle around it with a diameter of 0.5 cm.
00:23
Each quadrant has a point where the test chemical or control chemical will be deposited. It is important that the four points are equidistant from each other and from the center. At minimum, they must be 2 cm away from the center of the plate. Wet the plate with 1 mL of M9 buffer, making sure to evenly distribute around the plate.
00:44
You then add the worms to a 1.5 mL vial and allow them to settle at the bottom. Deposit the worm palette into the center of the plate and incubate for 1 hour at 20 degrees Celsius. At the end of the hour, count the number of worms in each quadrant that have completely crossed the central circle,
01:00
and use your data to calculate the chemotaxis index.