Pathomorphological Characteristics of Meningioma Biomineralization
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| License | CC Attribution 3.0 Germany: You are free to use, adapt and copy, distribute and transmit the work or content in adapted or unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor. | |
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Computer animation
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Computer animation
Transcript: English(auto-generated)
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Good evening dear ladies and gentlemen. My name is Anastasia Dinesenko and I am a first-year PhD student at the Department of Pathology of Sumo State University in Ukraine. It's a great honor for me to be here. Allow me to offer you a report on the topic pathomorphological characteristic of meningioma biomineralization. Meningiomas are most common primary tumors of central nervous
00:25
system characterized by calcification. It's more typical for women 40, 60 years old. And on the one hand calcification can create additional complication in diagnosis. On the other hand we can use it to improve differential diagnosis in this complex issue.
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We want to compare meningioma tissue with the normal one and in this case the best option is the duramata. Calcification of the duramata more typical for men with an average age 50 years and can be manifestation of age-related changes. However calcification of the duramata
01:02
in young population should be a reason for differential diagnosis with the tumors, consequences of subdural and epidural hemorrhages and calcium phosphorometabolism disorders. So the focus of our work is to study morphological features of calcification in meningioma tissue and the duramata tissue and in future it will create a basis for
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development a new material for biomedicine and can improve early diagnosis and treatment of central nervous system tumors. So six displacement of meningioma obtaining during neurosurgical interventions and six displacement of the duramata obtaining during autopsy
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at some original hospital were involved in our research protocol. We investigated them using the following methods. Microscopic examination, hematoxylene and azine staining, histochemistry, immunohistochemistry, immunofluorescent microscopy, scanning
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electron microscopy with energy dispersive x-ray spectroscopy, transmission electron microscopy. In future we also plan to use Raman spectroscopy, photo optometry, western blot and immunodot. So we remove tissue and fix it in 10% formalin solution for 24 hours.
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After that we cut strips from them, dehydrated and saturated with paraffin. Paraffin blocks were sectioned with a sinker, seven microns with a rotary microtome. Microscopic examination did not provide official visual information about presence of bio-minerals. So all samples after the paraffinization and dehydration were staining with hematoxylene and
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azine. In accordance with histological investigation we split our samples into four groups. Meningioma with calcification, meningioma without calcification, duramata with calcification and duramata without calcification.
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Staining with red alizarin method confirms the presence of calcium compounds in this tissue. Staining with Phongosa method confirms the presence of calcium phosphat in this tissue. And staining with Vangisone method confirms the presence of collagen fibers in this tissue.
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In accordance with scanning electron microscopy we can say that calcification are represented by rounded inclusion. And in case of the duramata it's from 10 nanometers to 50 microns. In meningiomas it's for 20 microns to 200 microns, so much larger.
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And in accordance with the element distribution map and the ratio between calcium and phosphorus we can say that it's calcium phosphat in form of hydroxyapatite. And this fact and size difference can be a biomarker for early diagnosis between normal tissue
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and tumor tissue. And also the futures of duramata can be a basis for development a nanomaterial for regenerative medicine and can improve treatment of central nervous system. Thank you for your attention.