Recombinant DENV 2 NS5: An Effective Antigen for Diagnosis of Dengue Infection
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| License | CC Attribution 3.0 Unported: You are free to use, adapt and copy, distribute and transmit the work or content in adapted or unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor. | |
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Transcript: English(auto-generated)
00:15
I've known everyone. I feel really honored to stand here. And the subject of my presentation
00:23
is the recombinant dengue virus to NS5, an effective antigen for diagnosis of dengue infections. Dengue virus belongs to flavor diol families and are classified in two, four
00:43
immunologically distinct serotypes can cause disease ranging from nonspecific fever illness to classic dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. Among the DHF and the DSS are often fatal. And dengue fever is endemic in tropic and
01:10
climates. There are nearly 390 million dengue virus infections occur annually. And over the past decades, dengue fever has separated from less than nine to currently about 128
01:26
academic countries. Dengue virus is transmitted by female mosquitoes, mainly age, aegypti, and angiopictus. All the stereotypes can be transmitted from human to human.
01:48
And individuals infected by any one stereotype will have lifelong immunity against re-infection of the same stereotype, but only partially protected from other stereotypes.
02:02
Infections with a different serotype are associated with a higher risk of severe complications such as DHF and the DSS due to ADE infection. And the dengue virus is also
02:21
a prototype of other flu viruses. For example, the Zika virus, this homology raises the risk of cross-reactive immune responses and subsequent ADE infections in the case of secondary infections
02:41
or immunization of the individuals. And the recent outbreak of Zika virus epidemics has raised a growing concern with both viruses. Around early 2016, the first dengue vaccine has been marketed in several countries, but
03:05
accumulating reports indicate that this vaccine did not achieve the design effect. And it should be conscious that this vaccine might cause ADE effect in populations.
03:33
Here are some prevention and control strategies proposed and practiced in dengue endemic regions,
03:42
but none of them achieved the effect. Dengue diagnosis is based on epidemic information, clinical symptoms, and combined laboratory detections. Although there is no specific treatment, early diagnosis is important for proper medical care to minimize mortality and
04:05
for proper initiation of public healthy control measures. There are several approaches to establish laboratory diagnosis, and these are of different specificity and sensitivity and require more than
04:29
two detection indicators. Any single indicator is not enough to provide reliable and effective results. And there are some rapid diagnosis of antigen detection and antibody detection
04:54
targeting NS1 are now commercially available, but both of them require all the four serotypes
05:04
or recombinant NS1. Why are we interested in NS5? Dengue virus NS5 are highly conserved among serotypes that have not been found to participate in the ADE phenomenon.
05:21
Dengue single open reading framework includes a polyprotein precursor, which is created by cellular and viral proteins in two structural and nonstructural proteins. NS1 and NS5 appear almost at the same time, and NS5 consists of two major functional domains,
05:49
the N-terminal I-methyltransferase domain and the C-terminal I-dependent I-polymerase domain. And the NS5-IDRP domain plays a vital role in viral RNA replications like
06:11
other polymerases. Dengue virus RNA processes an architecture that is conserved across different classes and families of polymerases. This structure mimics a half-closed right hand
06:27
with three subdomains termed finger, palm, and thumb. NS5 potential to be an antiviral target and a biomarker of dengue diagnosis. We constructed an NS5-IDRP domain
06:49
expression plasmid and an NS5-4 length expression plasmid and expressed a purified
07:03
70 kilo fragment and NS5-4 length fragment in E. coli. And these two purified recombinant
07:23
products reacted with sero of patients infected with dengue virus in an elastin with higher OD index compared with the control sera. And the recombinant dengue virus 2NS5
07:44
showed strong reactivity for any of the four serotypes, while the 70 kilo fragment shows strong reactivity for dengue virus 2 and 4. And dengue virus 2NS5 might be an effective
08:02
antigen for dengue diagnosis and the 70 kilo fragment also can be used for auxiliant diagnosis. Thank you.