Single-molecule fluorescence excitation spectroscopy

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Video in TIB AV-Portal: Single-molecule fluorescence excitation spectroscopy

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Title
Single-molecule fluorescence excitation spectroscopy
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CC Attribution - NoDerivatives 4.0 International:
You are free to use, copy, distribute and transmit the work or content in unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor.
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Release Date
2012
Language
English

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Subject Area
Abstract
The video demonstrates how the coupling of quantum emitters, such as single nanoparticles, to an external electromagnetic field can be investigated. An experimental configuration is presented, which employs a tunable supercontinuum white-light laser as an excitation source. A grating monochromator facilitates the selection of one wavelength at a time as well as its guidance to a confocal fluorescence microscope. After an isolated emitter is localized by raster scanning, a highly diluted sample prepared on a glass cover slip can be individually addressed and its excitation spectrum can be recorded. This allows for the detection of previously concealed quantum properties. Furthermore, the technique can be applied to sensitively study complex energy-migration processes in biological systems.
Keywords photo reactions
Physical chemistry Spectroscopy Cell (biology) Freies Molekül Fluorescence Spectroscopy
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Emission spectrum Molecule Sample (material) Quantum chemistry Solution Man page
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Sample (material) Emission spectrum Molecule Aage
Molecule Emission spectrum Nanoparticle Stockfish
Emission spectrum Sample (material) Spectroscopy Chemical plant Electronic cigarette Process (computing) Hope, Arkansas
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the university of tb in the physical chemistry laboratory for lazy spectroscopy easing molecules spectroscopy in all six my name in a cell for the next and professor here in the goal of our.
overseas to combine lasers spectroscopy with high resolution microscopy the goal is to investigate or to always have to watch molecules a turk this could be for example protein molecules are down to living cell phenomena in life sciences order. this could be surfaces or semiconductors for new kinds of optical computers are sensors or simply to understand or to learn to understand the interaction of light in matter and molecules and of course having fun when you do our work will show you. that at this specific example in a laboratory for me to electing like you.
the eye and frank i'm working as a generic leader in free next lap and i want to introduce in our experiments on single molecule fluorescence expectations spectroscopy conventional present studies are use one single one of chromatic were frantic so.
a sample and then are they wanted to or are the properties of the image of light and this is nice and this is well known but this is only part of the story because this is the way down and we were interested in the way out so how did the molecule couple to an external electromagnetic field and this is what we call expectations for.
just a p when we check which were flying gives you what our presence a mission and this is something which is comparable to solvents but it's on spectroscopy you won't be able to do arm with a single molecule because the signal to noise ratio would be much to look so we went another way was set up.
can we do this where fluorescence would take one single emission way fling and simply scan the expectation were five or broad range from the louis to the wrecked and to do so you need basically two requirements you need a very stable very bright white lace of source and you need a device to.
select one wavelength at a time and fortunately nowadays very intense super continuum lasers are available which we also used and the second thing is we have to select the way for length one way of playing for time and for that we used to grating spectrograph.
when we have. white lead come in and direct the wildlife to a grating and the grating just splits you white light into a rainbow and by these it selects a spectral colors and then we take this rainbow and directed to an entrance pinhole here and i simply turn the grading week.
can select one waiting at a time. the.
this which on the laser and when you see is a white light making the spot on the rating and are now you see the great and does its job and it spreads to live to to have this is wonderful rainbow here and now i switch on the motor to.
to turn the grating and we will seize that are step by step on the rainbow moves and we can select one way of things after the other.
they see. we stood in the blue go where the green yellow the red and up with a dark red to license but i blow some nitrogen steve over it.
the.
and you can honestly see the layers of different colors this is exactly what we want wanted to do two things with to synchronize the changing the way of playing with outdated with russian and for sure with to couple this in in to conform microscope there we can scan a sample address single molecules and.
the record or its attention spectrum.
a man and sebastian i'm going to show you hope to the measurements of what prank has told you before and for this week first of all sample of which consists of single molecules your of his arm we apply a solution of highly dilute the so-called quantum dots all.
on a rotating card last slip in a way that signal hugh dots are separated on this current last. next the wall we need some emerging loyal and apply it to the object of land microscope.
the.
before putting the some people. on the objective lance when it doesn't stick to power phone.
after this we need to exclude some room light because singing we are collecting from single molecule is very weak.
next to fall we need to focus sample he said sgt six.
it's.
one after switching off and i we can start measurement.
what you see here is to demand an old lessons intensity image of the sample and seven was moved there with the objective arm pixel wise a single molecule then shows up at such a bright spot and which can later be addressed taking spectrum.
from our measurement we obtain single molecule presses expectations spectrum of our nanoparticles and we find that the overall shape is more or less the same but we have distinct differences in the probability to go too long a duck states and if we just a grand the stock states we find there.
we have pronounced way flank where we have enhanced probability to go to dock states and the these finding is concealed in the awesome before some studies because he asked to go to individual emitters and to analyze them and this is only possible with a new technique and if you want to go step further you can for sure.
take these expectations spectroscopy for spectral imaging for example of biological samples and you would be able to money toward energy terms fair process is in for example plant closure of class or whatever you might think about and yeah this brings me to the end and a thank you for your attention.
and i hope you enjoyed watching the movie by.
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