The spectroscopic study of biomolecules in fruit and vegetables was increasingly attracting the interest of scientists around the world. Our work aims to use photoacoustic spectroscopy as a tool for studying biomolecules and optical characterization of organic materials in view of their ability to probe materials without the primary need to perform an extraction procedure. To this end, new computational systems and methods have been developed that allowed the execution, hardware automation, acquisition of data from the experiment and a better interpretation of them. The samples of interest were characterized by photoacoustic spectroscopy, and the results going to be analyzed using the second derivative method and the phase method. As future perspectives, the thermal properties of these materials going to be studied.