Recent observations of the cosmic microwave background at smallest angular scales and updated abundances of primordial elements indicate an increase of the energy density and the helium-4 abundance with respect to standard big bang nucleosynthesis with three neutrino flavour. This calls for a reanalysis of the observational bounds on neutrino chemical potentials, which encode the number asymmetry between cosmic neutrinos and anti-neutrinos and thus measures the lepton asymmetry of the Universe. We compare recent data with a big bang nucleosynthesis code, assuming neutrino flavour equilibration via neutrino oscillations before the onset of big bang nucleosynthesis. We find a preference for negative neutrino chemical potentials, which would imply an excess of anti-neutrinos and thus a negative lepton number of the Universe. This lepton asymmetry could exceed the baryon asymmetry by orders of magnitude.