FIB-SEM video composed of 501 electron backscatter images. After each image a section of 10 nm was removed and a new section was imaged. The video shows two filaments with a horizontal cross-sectional view along the length of the filament that can be seen on the right at the start of the video. The filament that is visible on the left at the start of the video follows a different direction and therefore the cross-section shows different angles. The thick layer of mineral encrustation appears as a dark seal around the filament, the ridge compartments and the cell content appear white. The encrustation is only found around the filament and not within the periplasmic space. Both filaments are strongly encrusted and the mineral crust of both filaments is partly cemented together which can be observed from 0:06 till 0:26. The encrustation is heterogeneous with an average thickness of ˜500 nm.