Studying And Fighting Pathogenic Bacteria with the Help of Crispr
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| Title | Studying And Fighting Pathogenic Bacteria with the Help of Crispr |
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| Number of Parts | 38 |
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| License | You are free to use, adapt and copy, distribute and transmit the work or content in adapted or unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor. |
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| Abstract | Cas systems have emerged has a powerful biotechnological tool. The Cas9 protein is a RNA-guided nuclease that can be easily reprogrammed to target any sequence of
interest. Our work focuses on the development of CRISPR-Cas9 tools to edit bacterial genomes and control gene expression. In particular, we investigate how these tools can be used in high-throughput screens to perform functional screens. Recently we have also shown how CRISPR system can be used as sequence-specific antimicrobial. The Cas9 protein can kill bacteria when directed to cut in their chromosome. Guide RNAs can be programmed to kill Bacteria carrying antibiotic
resistance or virulence genes specifically, and the CRISPR system can delivered to bacterial populations using phage capsids. |
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