UCI Chem 128 Introduction to Chemical Biology (Winter 2013) Instructor: Gregory Weiss, Ph.D. Description: Introduction to the basic principles of chemical biology: structures and reactivity; chemical mechanisms of enzyme catalysis; chemistry of signaling, biosynthesis, and metabolic pathways. Index of Topics: 0:02:04 Enzyme Functions 0:06:10 Serine Based Proteases 0:10:44 Protein Based Inhibition of Proteases 0:13:10 Covalent or Mechanism-Based Protease Inhibitors 0:15:02 Inhibition of Serine Esterases 0:17:07 Enzymes Use Co-Factors (Vitamins) 0:21:31 The Origins of Stereospecificity in Alcohol Dehydrogenase 0:24:09 Pyridozal Phosphate (Vitamin 86) 0:27:29 PLP - Catalyzed Transamination 0:29:29 Protein Engineering 0:36:16 Most Mutations Make the Protein Less Functional 0:38:17 Carbohydrates 0:44:30 Hemiacetal Reactivity and Formation 0:46:33 Glucopyranose is the Most Noteable Ring Configuration 0:47:51 Oligosaccharides of the TB Coat 0:51:29 Oxocarbenium Ions as a Key Intermediate in Hydrolysis of Glycosidic Bonds 0:53:19 Mechanisms of Enzymatic Hydrolysis 0:54:58 Commonalitites in Glycosylhydrolase Mechanisms 0:56:03 Neuraminidase: Key Enzyme in Influenza Release from Surface to Cell 1:01:06 Oligosaccharides 1:04:22 Polysaccharides 1:08:04 Hyaluronan: Oligosaccharides in Joints 1:09:57 Glycosylated Proteins