UCI Chem 128 Introduction to Chemical Biology (Winter 2013) Instructor: Gregory Weiss, Ph.D. Description: Introduction to the basic principles of chemical biology: structures and reactivity; chemical mechanisms of enzyme catalysis; chemistry of signaling, biosynthesis, and metabolic pathways. Index of Topics: 0:15:32 Protein Conformation 0:18:28 All B-Sheet Proteins 0:27:13 WD Proteins Scaffold Together Large Assemblies 0:28:12 Collagen is Formed from a 3-Stranded Coil 0:29:21 All a-Helical Proteins 0:31:40 a/B Proteins 0:33:21 Peptide Binding Domains 0:39:05 Higher Order Assemblies of Proteins 0:41:23 Equilibrium Constants to Describe the Strengths of Non-Covalent Interactions 0:46:54 Following the SPeeds of Reactions 0:49:25 Rates of Non-Covalent Interactions 0:50:16 Typical Rates of Binding 0:53:09 Measuring Biological Potency Through Dose Responsive Curve 0:56:26 Measuring Biological Reponse by ELISA 0:59:36 Steptavidin-Biotin Offers Near Covalent Binding Affinity 1:00:39 Biotinylated Reagents Used Extensively in Chemical Biology 1:03:47 Enxymatic Catalysts = Catalytic Receptors