Laubmoos. Versuche zur Bildung von Plasmolysetonoplasten: Zunächst normale Plasmolyse mit Deplasmolyse. In hypertonischer Kaliumrhodanid-Lösung wird das Plasmalemma geschädigt, und es kommt zur Tonoplasten-Plasmolyse. In schwächeren Lösungen treten Mischformen der Plasmolyse auf (sekundäre Tonoplasten-Plasmolyse). Mit Zeitraffung.

The film shows plasmolytic tonoplasts in leaf cells of the moss Hookeria lucens. The tonoplasts become visible after the cells are treated with a harmful solution which damages the plasmalemma, or also after multiple, rapid plasmolysis and deplasmolysis in harmless solutions which subject the protoplasts to mechanical stress. First, normal plasmolysis followed by a slower, non damaging deplasmolysis is shown. The cells can be again plasmolyzed in a harmless KCl solution without injury. Next, tonoplasts can be seen after KCNS-treatment, which is very harmful to the plasmalemma. Different effects can be observed can be observed by varying the strength of the KCNS solutions used. In strong solutions, tonoplasts appear immediately (primary tonoplast plasmolysis). In somewhat weaker solutions, one usually sees a normal plasmolysis; many cells plasmolyze normally at first, but after some time the KCNS destroys the plasmalemma, so that the surviving tonoplasts are visible (secondary tonoplast plasmolysis). The two last scenes show tonoplasts after damage of the plasmalemma by rapid deplasmolysis.