Graphene-based materials (GBMs) are promising nanomaterials for innovation in energy, health, sensors, and agri-environmental technologies. It is essential therefore to understand the impacts of these nanomaterials on biological and environmental systems towards safe and sustainable innovation. At the environment, GBMs will present different toxicological effects linked to its physico-chemical properties and mechanisms of nano-interactions with living organisms. GBMs will interact with biomolecules and pollutants modulating its fate and toxicological response. In this sense, it is very important to study the effects of GBMs on the mixture/combined toxicity considering co-exposure scenarios. In this talk, we will present an overview involving graphene oxide-based materials, transformations and single- and mixture ecotoxicity evaluations. Nanoinformatics emerges as key research area support the integration of data, modeling and risk prediction of GBMs; challenges and future perspectives will be presented for discussions by viewpoint of an experimental nano-ecotoxicologist.