Purpose: The study evaluated the accuracy of depth perception afforded by static and dynamic stereoscopic three-dimensional (S3D) images with proportional (scaled to disparity) and constant size cues. Methods: Sixty adult participants, 18 to 40 years (mean, 24.8 years), with good binocular vision participated in the study. For static S3D trials, participants were asked to indicate the depth of stationary S3D images rendered with 36, 48 and 60 pixels of crossed disparity, and with either proportional or a constant size. For dynamic S3D trials, participants were asked to indicate the time when S3D images, moving at 27, 32 and 40 pixels/sec, matched the depth of a reference image which was presented with 36, 48 and 60 pixels of crossed image disparity. Results: Results show that viewers perceived S3D images as being closer than would be predicted by the magnitude of image disparity, and correspondingly they overestimated the depth in moving S3D images. The resultant depth perception and estimate of motion speed were more accurate for conditions with proportional and larger image size, slower motion-in-depth and larger image disparity. Conclusion: These findings possibly explain why effects such as looming are over stimulating in S3D viewing. To increase the accuracy of depth perception, S3D content should match image size to its disparity level, utilize larger depth separation (without inducing excessive discomfort) and render slower motion in depth. © (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).