UCI Chem 128 Introduction to Chemical Biology (Winter 2013) Description: Introduction to the basic principles of chemical biology: structures and reactivity; chemical mechanisms of enzyme catalysis; chemistry of signaling, biosynthesis, and metabolic pathways. Index of Topics: 0:21:06 RNA and Transcription Factors 0:26:04 Comparing Bacterial and Eukaryotic mRNA Processing 0:29:08 CTP Cap Methylation 0:30:30 Using PolyA Tails to Isolate mRNA 0:34:29 Eukaryotic Splicing of mRNAs 0:37:38 RNA Degredation Plays a Major Role 0:40:40 Therapeutic Anti-Sense 0:42:32 Modifying the Oligo Backbone 0:44:20 RNA Interference Used Extensively in the Lab 0:47:57 Where Peptide Synthesis Starts 0:58:26 The Genetic Code: The Language of the Codons 1:00:33 Decoding the DNA to Protein Sequence 1:01:43 How to Load the Amino Acyl tRNA 1:06:38 Post-Translational Modification of the N-Terminus 1:07:25 Inhibiting Methionine Aminopeptidase 1:09:59 Binding to mRNA Provides Further Regulation of Translation 1:10:59 Incorporating Unnatrual Amino Acids 1:12:38 Expanding the Protein Palette 1:13:48 mRNA Aptamer Libraries 1:18:18 Puromycin Allows Covalent Linkage to the Growing Peptide During Translation