We're sorry but this page doesn't work properly without JavaScript enabled. Please enable it to continue.
Feedback

Genome-scale dissection of mammalian membrane trafficking - new players and unexpected mechanism

Formal Metadata

Title
Genome-scale dissection of mammalian membrane trafficking - new players and unexpected mechanism
Title of Series
Number of Parts
34
Author
License
CC Attribution 3.0 Unported:
You are free to use, adapt and copy, distribute and transmit the work or content in adapted or unchanged form for any legal purpose as long as the work is attributed to the author in the manner specified by the author or licensor.
Identifiers
Publisher
Release Date2018
LanguageEnglish

Content Metadata

Subject Area
Genre
Abstract
Cargo proteins moving between organelles are transported by membrane-enclosed vesicles. The core engines mediating vesicle trafficking are now well established. However, we areonly beginning to understand the regulatory networks superimposed upon the core engines to adjust the rate and direction of membrane transport according to physiological demands. The advent of the revolutionary CRISPR-Cas9 genome editing system enabled us to systematically identify new components of the regulatory networks. We developed new screening platforms and performed unbiased genome-wide CRISPR genetic screens to dissect the exocytosis and endocytosis of cell surface transporters, fundamental processes in cell physiology. Our screens identified known regulators but most of the hits were not previously known to regulate the pathways. I will focus on the unexpected mechanisms of RABIF/MSS4 in exocytosis and AAGAB in endocytosis. I will also discuss how the principles uncovered in our studies shed light on vesicle trafficking in general.
Keywords