Tutorials on STRENDA DB
4
2017
6
26 Minuten 36 Sekunden
4 Ergebnisse
03:44
3Kettner, CarstenBrief introduction… …of the Beilstein-Institut and STRENDA, the project that is concerned with the improvement of the quality of functional enzyme data in the literature. Carsten Kettner explains the background of this project and invites authors who are preparing a manuscript that contains functional enzymology data to learn more on how to ensure that their data sets are complete and valid before they are submitted as part of a publication to a journal. The STRENDA Commission has established standards for data reporting in enzymology research. The aim of these STRENDA Guidelines is to improve the quality of data published in the scientific literature and to enable researchers to compare, evaluate, interpret and reproduce experimental research results published in the literature and databases. STRENDA supports authors by providing STRENDA DB – a web-based database whose data submission form automatically checks the manuscript data prior or during the publication process on compliance with the STRENDA Guidelines.
2017Beilstein-Institut zur Förderung der Chemischen Wissenschaften
03:40
2Kettner, CarstenBasic Concept behind STRENDA DB Before entering example data, Carsten Kettner describes the basic concept behind STRENDA DB which reflects substantially the common structure of a manuscript. For the data input, STRENDA DB requires header data (the title of the manuscript and the name(s) of the author(s), data on the protein assayed, information on the methodology used and techniques applied including details on the assay conditions and results data that describe the kinetic activity of the enzyme. In a first step, it is demonstrated how to create a Manuscript in STRENDA DB including the input of the header data.
2017Beilstein-Institut zur Förderung der Chemischen Wissenschaften
07:38
1Kettner, CarstenDefinition of the term “Experiment” and input of protein data In order to generate a technical understanding of the term “Experiment” used throughout in STRENDA DB, Carsten Kettner explains its meaning: an “Experiment” is characterized by the protein assayed. One manuscript can contain one or more “Experiments” if for example the authors compared the catalytic activity of the native protein with a genetically modified one or two homolog proteins from different tissues, organs or organisms. Then, it is shown, how to define an experiment followed by the entries required to comprehensively describe the protein used in the assay.
2017Beilstein-Institut zur Förderung der Chemischen Wissenschaften
11:33
Kettner, CarstenConcept of “Experimental Subset” and input of assay conditions and results data In STRENDA DB an additional term is used, i.e “Experimental Subset” which is explained in details in order to generate the technical understanding required to navigate through the input process. An “Experimental Subset” can be considered a container for those data that define the assay conditions along with those data that define the results from the investigation of the functional activity of an enzyme. Since the enzyme kinetics is strongly dependent of the assay conditions at which the experiment has been carried out, assay conditions and results data are combined. One experiment can contain one or more “Experimental Subsets”. This is very often the case, e.g. when profiles are studied such as pH profiles, substrate specificities or the effect of various modifiers, i.e. inhibitors and activators. After the successful data entry, each experiment is assigned a STRENDA Registry Number (SRN) and a DOI. The conceptual introduction is followed by the entry of example data for the assay conditions and results.
2017Beilstein-Institut zur Förderung der Chemischen Wissenschaften