Grey literature is information produced outside of traditional publishing and distribution channels. Grey literature constitutes a wide range of sources such as clinical trials, blogs, tweets, newsletters that are difficult to track down for its heterogeneous format and diverse sources. There are a large number of publications from western countries in health sciences demonstrating that including grey literature in search enhances search results and provides useful evidence especially while conducting systematic reviews. However, in low- and middle-income nations, such as India they are insufficiently addressed in scientific publications, policy documents, theory and practice and hardly any paper found in public health discipline. Road traffic injuries are highly prevalent in India. We have taken road traffic injuries as a case study from India where we will identify grey material on road traffic injuries that are of relevance to public health practitioners and health administrators. We will conduct a grey literature search from a range of non-traditional sources for the term ‘road traffic injuries’ that are published from or based in India such as recent technical reports, conference proceeding papers, thesis and dissertations, papers in pre-print archives, websites available in English language that are published since 2015 and also government data on road traffic injuries. We will share best practices available in grey literature and those from other published scientific papers on road safety. Grey literature contains valuable information that supports research, facilitates decision making and advances education. They have far high relevance and significance with emergence of newer diseases and sophisticated technologies. The findings of this study will enhance access, the use of grey literature and in gathering of the evidence from multiple sources by a wide range of stakeholders in low-income countries.