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10:59 IWF (Göttingen) English 1984

Production of Purified Clostridia Toxoid Vaccines by Cascade Ultrafiltration

A system is described which consists firstly of identifying pathogen anaerobes in tropical grazing lands by means of GLC and biometrical comparison of metabolically produced fatty acid patterns with such of known reference strains. Locality specific anaerobes may then be continuously cultivated in an especially designed bioreactor and their toxins separated through ultra-filtration. The filtration process is a continuous two step procedure. At first the microbes are separated from the spent broth with a hollow fiber of approx. 1 Mio. NMCO and in the second stage the toxin is concentrated and freed of allergenic peptides with a hollow fiber filter of 20.000 NMCO. The highly concentrated formalinized toxoid may be applicated intradermically beside the tail of the animal in a volume of 0,25 ml with a dermo-jet.
  • Published: 1984
  • Publisher: IWF (Göttingen)
  • Language: English
09:46 IWF (Göttingen) German 1987

Interzelluläre Kommunikation über Gap junctions

Gap junctions dienen der Informationsübertragung in Geweben und Organen tierischer Herkunft. Sie bestehen aus Ansammlungen feiner Kanälchen, die einen direkten Kontakt zwischen benachbarten Zellen herstellen und so eine schnelle Nachrichtenübermittlung zulassen. Darstellung des Aufbaus einer gap junction in Trick- und EM-Aufnahmen. Demonstration der Kopplung von Zellen durch Ausbreitung von Fluoreszensfarbstoff (Lucifer yellow) und Übertragung elektrischer Signale.
  • Published: 1987
  • Publisher: IWF (Göttingen)
  • Language: German
1:01:46 IWF (Göttingen) German 1983

Blutfluidität als Folge der Erythrozytenfluidität

Bewegung von Erythrozyten in Kapillaren der Ratte und des Frosches (Original). Entwicklung des Erythrozyten aus dem Erythroblasten (Trick). Demonstration der Fließfähigkeit von gehärteten und unbehandelten Erythrozyten sowie einer konzentrierten Erythrozytensuspension (Original).Physikalische Bedingungen des Fließens (Scherung, Schubspannung, Schergeschwindigkeit). Entstehung laminarer Strömung (Trick). Beobachtung von Erythrozyten im gegenrotatorischen Koaxialzylinder-Rheometer. Nachweis von Orientierung, Deformierung, Membranrotation und Innenzirkulation der Erythrozyten (Original, Trick). Fließverhalten von Erythrozyten in Glas- und physiologischen Kapillaren (Original).
  • Published: 1983
  • Publisher: IWF (Göttingen)
  • Language: German
13:00 IWF (Göttingen) English 1979

Post-Ischemic Acute Renal Failure (Tupaia belangeri, Rattus norvegicus)

Normal function of the nephrons shown with microcinematography; illustration of the normal and pathological conditions of the nephrons with histological sections and vital microscopy; schematic drawings compliment microscopic findings. Ischemia causes epithelial damage in the nephrons and leads to the formation of proteinaceous casts, which prevent normal flow of urine and cause renal failure.
  • Published: 1979
  • Publisher: IWF (Göttingen)
  • Language: English
05:42 IWF (Göttingen) English 1977

Structure of the Blood Vessels and Circulation of Blood in the Glomus caroticum of the Cat

Some passages of the dye Lissamin green: fluorscent shots. The cat carotid body is a chemoreceptor which measures the partial pressures of oxygen and carbon dioxide, and pH values in blood plasma. This organ seperates the corpuscles from the serum so that the receptors measure only values of plasma. Morphology and function are shown.
  • Published: 1977
  • Publisher: IWF (Göttingen)
  • Language: English
05:25 IWF (Göttingen) German 1965

Lissamingrün-Passagen in der Nierenpapille - Mesocricetus auratus (Muridae)

The film shows the passage of lissamin green in the renal papilla of a golden hamster (dye passage through vasa recta, loops of Henle, and collecting ducts). Particular attention is paid to the rhythmic transport of dye in the collecting duct system. Furthermore the passage of albumin cylinders in the collecting duct system is shown, whereby the effect of osmotic diuretics is demonstrated.
  • Published: 1965
  • Publisher: IWF (Göttingen)
  • Language: German
22:11 IWF (Göttingen) English 1983

Fluidity of Blood as a Consequence of Fluidity of Erythrocytes

Movement of erythrocytes in the capillaries of the rat and of the frog (original image). Development of erythrocytes from an erythroblast (special effects). Demonstration of the fluidity of hardened and untreated erythrocytes as well as a concentrated erythrocyte suspension (original image): Physical conditions of flow (shear, shear stress, shear rate). Development of laminar flow (special effects). Observation of erythrocytes in counter-rotatory coaxial-cylinder rheometer. Demonstration of orientation, deformation, membrane rotation and internal circulation of erythrocytes (original image, special effects). Flow behaviour of erythrocytes in glass and physiological capillaries (original image).
  • Published: 1983
  • Publisher: IWF (Göttingen)
  • Language: English
13:00 IWF (Göttingen) German 1979

Postischämisches akutes Nierenversagen (Tupaia belangeri, Rattus norvegicus)

Normal function of the nephrons shown with microcinematography; illustration of the normal and pathological conditions of the nephrons with histological sections and vital microscopy; schematic drawings compliment microscopic findings. Ischemia causes epithelial damage in the nephrons and leads to the formation of proteinaceous casts, which prevent normal flow of urine and cause renal failure.
  • Published: 1979
  • Publisher: IWF (Göttingen)
  • Language: German
05:26 IWF (Göttingen) German 1965

Lissamingrün-Passagen in der Nierenrinde - Rattus norvegicus (Muridae)

After showing the normal, decapsulated kidney surface of an anaesthetized albino rat, the film shows the passage of intravenously injected Lissamingreen on the kidney surface. The dye passes through the vasa efferentia, the proximal and the distal tubulus lumina at characteristic times, thereby permitting - in addition to the differentiation of proximal and distal tubuli - the determination of tubular flow rates. This is followed by a description of the behaviour of the proximal and distal tubuli lumina during interruption of glomerular filtration by clamping the aorta.
  • Published: 1965
  • Publisher: IWF (Göttingen)
  • Language: German
04:22 IWF (Göttingen) German 1971

Durchblutungsänderungen der Nierenoberfläche der Ratte unter Sympathicusreizung

Pallor due to localised vasoconstriction. Severity and spread of the effect (Lissamine green passages).
  • Published: 1971
  • Publisher: IWF (Göttingen)
  • Language: German
04:11 IWF (Göttingen) German 1971

Tubuläre Resorption an der Nierenoberfläche der Ratte (Split drop)

Injection of a contrast medium-oil mixture into a proximal convoluted tubule. Division of the dye column by an intermediate injection of a test solution. Co-joining of the dye columns as a measure of the test solution resorption.
  • Published: 1971
  • Publisher: IWF (Göttingen)
  • Language: German
05:42 IWF (Göttingen) German 1977

Durchblutung und Gefäßaufbau des Glomus caroticum der Katze

Lissamingrün-Passagen. Fluoreszenzaufnahmen. Glomus caroticum ist ein Chemorezeptor, welcher im Blutplasma Sauerstoff- und Kohlensäuredruck und den pH-Wert mißt. Das Organ hat Trennfunktion zwischen korpuskulären Anteilen des Blutes und dem Serum. Morphologie und Funktion werden gezeigt. Mit Trick.
  • Published: 1977
  • Publisher: IWF (Göttingen)
  • Language: German
05:06 IWF (Göttingen) English 1971

Glomerular Filtration and Microcirculation in the Kidney of the Rat

Direct observation of the passage of contrast medium in the glomeruli, capsular area and distal convolute filmed at various speeds.
  • Published: 1971
  • Publisher: IWF (Göttingen)
  • Language: English
04:39 IWF (Göttingen) Silent film 1950

Kreislaufstudien an Herz und großen Venen (Tierversuche)

Ventricular movements, blood flow in heart cavities and heart vessels (slow motion x-ray cinematography).
  • Published: 1950
  • Publisher: IWF (Göttingen)
  • Language: Silent film
03:40 IWF (Göttingen) Silent film 1950

Kreislaufstudien an Herz und großen Arterien (Tierversuche)

Change of shape of heart cavities, blood flow in aorta, larges arteries, large veins and heart (slow motion x-ray cinematography).
  • Published: 1950
  • Publisher: IWF (Göttingen)
  • Language: Silent film
17:53 IWF (Göttingen) English 1994

Experiments on Single Ventricular Myocytes

The central topic is the modification of the respective action potentials or ion flows of isolated myocards under various experimental conditions. The following issues are considered: the patch-clamp and voltage-clamp techniques (animation); origination of the action potential (animation); the effects of cadmium and adrenaline (action potentials, ion flows, optical cinemicrography); the interpendency between action potential and stimulation frequency or the absolute and relative refractory period, respectively (with simultaneous display of cinemicrographs and action potentials).
  • Published: 1994
  • Publisher: IWF (Göttingen)
  • Language: English
18:21 IWF (Göttingen) English 1992

Experiments on the Physiology of Heart and Circulation of the Rabbit

The experiments conducted in the closed thorax show several examples of control mechanisms of the cardiac and circulatory functions. Accompanied by consistent monitoring of the ECG, pulse frequency and blood pressure, the following interventions are performed: 1. Simultaneous ligature of the carotids, 2. Separate electrostimulation of the vagus nerves, 3. Intravenous injection of acetylcholine (neurotransmitter), adrenaline (hormone) and Verapamil (medicament, calcium antagonist).
  • Published: 1992
  • Publisher: IWF (Göttingen)
  • Language: English
14:02 IWF (Göttingen) English 1992

Experiments on the Isolated Heart of the Rabbit in the Langendorff-Apparatus

Following an introduction to the experimental set-up (partial animation), the still beating heart is isolated from the thorax and perfused with solutions of adrenaline, acetylcholine and verapamil. The changes in contractile force and pulse frequency are displayed graphically and elucidated. In conclusion, cooling of the carotid sinus (the primary pacemaker) and a demonstration of the typical consequences.
  • Published: 1992
  • Publisher: IWF (Göttingen)
  • Language: English
17:41 IWF (Göttingen) German 1994

Experimente an isolierten Zellen des Ventrikelmyokards

Zentrales Thema sind die Veränderungen der Aktionspotentiale bzw. der Ionenströme von isolierten Herzzellen unter verschiedenen experimentellen Bedingungen. Im einzelnen werden behandelt: das Patch-Clamp- und Voltage-Clamp-Verfahren (Trick), die Entstehung des Aktionspotentials (Trick), die Wirkung von Cadmium und Adrenalin (Aktionspotentiale, Ionenströme, lichtmikroskopische Aufnahmen), die Abhängigkeit der Aktionspotentiale von der Stimulationsfrequenz bzw. der absoluten und relativen Refraktärzeit (gleichzeitige Darstellung von lichtmikroskopischen Aufnahmen und Aktionspotentialen).
  • Published: 1994
  • Publisher: IWF (Göttingen)
  • Language: German
05:53 Medizinische Hochschule Hannover (MHH), Exzellenzcluster REBIRTH German 2012

Künftige Methoden für das Tissue Engineering am Laserzentrum Hannover

  • Published: 2012
  • Publisher: Medizinische Hochschule Hannover (MHH), Exzellenzcluster REBIRTH
  • Language: German
03:50 Medizinische Hochschule Hannover (MHH), Exzellenzcluster REBIRTH English 2012

Cryopreservation or: how can I extend the shelf life of cells?

  • Published: 2012
  • Publisher: Medizinische Hochschule Hannover (MHH), Exzellenzcluster REBIRTH
  • Language: English
03:51 Medizinische Hochschule Hannover (MHH), Exzellenzcluster REBIRTH German 2012

Kryokonservierung oder: Wie hebt man Zellen am besten auf?

  • Published: 2012
  • Publisher: Medizinische Hochschule Hannover (MHH), Exzellenzcluster REBIRTH
  • Language: German
16:04 Institut für Film und Bild in Wissenschaft und Unterricht, Abteilung Hochschule und Forschung (Göttingen) Silent film 1950

Indianerleben im Gran Chaco

Grass hut settlement; digging stick construction; house building; preparation of food; manufacture of double-string bow; hunt; fishing; men's festival; medicine man treating ill; tieing knots; spinning and weaving; row and circle dances of women. Recording speed 16 fps, projection speed 18 fps.
  • Published: 1950
  • Publisher: Institut für Film und Bild in Wissenschaft und Unterricht, Abteilung Hochschule und Forschung (Göttingen)
  • Language: Silent film
05:53 Medizinische Hochschule Hannover (MHH), Exzellenzcluster REBIRTH English 2012

Future methods of tissue engineering at Hannover Laser Centre

  • Published: 2012
  • Publisher: Medizinische Hochschule Hannover (MHH), Exzellenzcluster REBIRTH
  • Language: English
03:28 IWF (Göttingen) Silent film 1961

Skelettmuskulatur - Kontraktionswellen in Einzelfasern - Geotrupes silvaticus (Geotrupidae)

Dor beetle. Course of spontanously emerged contraction waves in skeletal muscle fibres (slow-motion).
  • Published: 1961
  • Publisher: IWF (Göttingen)
  • Language: Silent film
Offline Collection IWF (Göttingen) Silent film 1978

Azande (Äquatorialafrika, Nordost-Zaire) - Traditionelle Behandlung einer Patientin mit einer Thoraxerkrankung

By massage and rubbing with a handful of fibrous material from the inner bark of a particular plant the practitioner "extracts" the disease from the thorax of a woman in the form of small splinters of bone. In this way he removes altogether 11 small splinters, preventing them afterwards from getting back to the woman's upper body by anointing it with healing oil. In order to detect the guilty person, i. e. the person having caused the disease by special spiritual powers and abilities, the practitioner carries out a sand-oracle, which reveals the husband's mistress as responsible for the illness.
  • Published: 1978
  • Publisher: IWF (Göttingen)
  • Language: Silent film
Offline Collection IWF (Göttingen) Silent film 1978

Azande (Äquatorialafrika, Nordost-Zaire) - Traditionelle Behandlung von Patienten mit rheumatischen Beschwerden

The practitioner chars a special kind of wood over the fire, reduces it to powder, mixes the powder on a special potsherd with healing oil, and rubs the resulting black paste into incisions cut previously with an old blade made in Europe in the patient's skin.
  • Published: 1978
  • Publisher: IWF (Göttingen)
  • Language: Silent film
06:10 IWF (Göttingen) English 1983

Artificial Insemination in Cattle - 3. Insemination

Explanation of oestrous symptoms. The use of stud bull to recognize ovulation time. Menstrual calender. Examination of the external genitalia. Preparation of the semen. Introduction of the inseminator into the vaginal opening and internal examination. Release of sperm into the cervical canal (animation). Hygiene.
  • Published: 1983
  • Publisher: IWF (Göttingen)
  • Language: English
17:13 IWF (Göttingen) German 1977

Aufbau und Funktion des Skelettmuskels

A single muscle cell is dissected out of the m. semitendinosus from the frog's leg. Demonstration of the threshold stimulus and response behaviour, two single contractions superimposed on one another and a tetanus, experimental determination of the length-force relationship and the relationship between speed of contraction and load.
  • Published: 1977
  • Publisher: IWF (Göttingen)
  • Language: German
12:47 IWF (Göttingen) English 1971

Fertilization and Early Development of the Mammalian Egg (Rabbit)

Animation sequences of: microscopic anatomy of the Graafian follicle, transport and fertilization in the oviduct. Cinemicrography partly in time-lapse of rabbit ovum: fertilization, fusion of pronuclei, first mitosis, subsequent cleavages (blastula, morula, blastocyst).
  • Published: 1971
  • Publisher: IWF (Göttingen)
  • Language: English
10:15 IWF (Göttingen) German 1993

Makrophagen - HIV-induzierte Synzytienbildung bei humanen Monozyten/Makrophagen

Synzytienbildung durch Zellfusion nach Infektion mit HIV. Humane Blutmonozyten reifen in der Zellkultur zu Makrophagen. Endomitosen treten auf. 10 bis 13 Tage nach In-vitro-Infektion mit humanem Immunodefizienz Virus Typ 1 (HIV-1) bilden sich mehrkernige Riesenzellen unter Beteiligung verschieden differenzierter Makrophagen. Der zytopathische Effekt hat Ähnlichkeit mit neurohistopathologischen Befunden. Mikrokinematografie mit Zeitraffung sowie Trickbearbeitung.
  • Published: 1993
  • Publisher: IWF (Göttingen)
  • Language: German
18:21 IWF (Göttingen) German 1992

Experimente zur Physiologie von Herz und Kreislauf am Kaninchen

Die Experimente, die bei geschlossenem Thorax durchgeführt werden, zeigen einige Beispiele für Steuermechanismen der Herz- und Kreislauffunktionen. Unter ständiger Beobachtung von EKG, Herzfrequenz und Blutdruck werden folgende Eingriffe vorgenommen: 1. Gleichzeitige Abklemmung der Karotiden. 2. Getrennte elektrische Reizung der Nervi vagi. 3. Injektion (i. v.) von Acetylcholin (Neurotransmitter), Adrenalin (Hormon) und Verapamil (Medikament, Kalziumantagonist).
  • Published: 1992
  • Publisher: IWF (Göttingen)
  • Language: German
14:00 IWF (Göttingen) German 1992

Experimente am isolierten Kaninchenherzen in der Langendorff-Apparatur

Nach einer Einführung in die Versuchsanordnung (z. T. Trick) wird ein aus dem Thorax isoliertes und weiterhin schlagendes Herz mit Lösungen von Adrenalin, Acetylcholin und Verapamil perfundiert. Die Veränderungen von Kontraktionskraft und Schlagfrequenz werden als Kurven aufgezeichnet und besprochen. Abschließend Kühlung des Sinusknotens (primärer Schrittmacher) und Demonstration der typischen Folgen.
  • Published: 1992
  • Publisher: IWF (Göttingen)
  • Language: German
05:47 IWF (Göttingen) German 1977

Präparation einer einzelnen Skelettmuskelzelle

A single fibre from a frog semiteninosus muscle is dissected in a special dissection dish. The main stages of this procedure are as follows: about 2/3 of the fibres are cut off by using fine scissors, the connective tissue in the remaining part of the preparation is made loose by stretching and pulling, the preparation is subdivided in small fibre bundles, one bundle is chosen for final dissection, and the single fibre is isolated from its last fellow fibres. Finally, the isolated fibre is tested for all-or-nothing twitches.
  • Published: 1977
  • Publisher: IWF (Göttingen)
  • Language: German
12:59 IWF (Göttingen) English 1988

Biotechnics in Reproduction - Micromanipulation of Oocytes and Embryos in Mice

Development of a fertilized mouse egg up to blastocyte. Micro-surgery; injection of DNA. Production of a chimera and of genetically identical twins.
  • Published: 1988
  • Publisher: IWF (Göttingen)
  • Language: English
21:03 IWF (Göttingen) German 1988

Biotechnik in der Fortpflanzung - Embryotransfer und Mikromanipulation beim Rind

Gewinnung und Übertragung von Embryonen nach hormoneller Vorbehandlung; Tiefgefrierkonservierung der Embryonen; Mikromanipulation: Zwillingserstellung und Chimärenerzeugung.
  • Published: 1988
  • Publisher: IWF (Göttingen)
  • Language: German
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