Laser Microbeam and Optical Tweezers - Micromanipulation of Gametes and Embryos

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Formal Metadata

Title
Laser Microbeam and Optical Tweezers - Micromanipulation of Gametes and Embryos
Alternative Title
Lasermikrostrahl und optische Pinzette - Mikromanipulation an Gameten und Embryonen
Author
Schütze, Karin
Clement-Sengewald, A.
Berg, F. D.
Brehm, G.
Schütze, R.
Contributors
Gertraude Kerlen (Redaktion)
Jürgen Kaeding (Kamera)
Siegfried Umbreit, Abbas Yousefpour (MAZ-Technik und Schnitt)
License
CC Attribution - NonCommercial - NoDerivatives 3.0 Germany:
You are free to use, copy, distribute and transmit the work or content in unchanged form for any legal and non-commercial purpose as long as the work is attributed to the author in the manner specified by the author or licensor.
DOI
IWF Signature
C 1921
Publisher
IWF (Göttingen)
Release Date
1996
Language
English
Producer
IWF
Production Year
1995

Technical Metadata

IWF Technical Data
Video ; F, 16 min

Content Metadata

Subject Area
Abstract
The practical applications of contact-free laser micro-manipulation to in vitro fertilization are demonstrated. The UV laser microbeam and IR laser optical tweezers are shown in conjunction with an optical microscope. The techniques illustrated include use of laser scissors for blastomere fusion in murine 2-cell embryos, opening of the oocyte zona pellucida to facilitate sperm penetration and immobilization of sperm by tail excision. The use of tweezers is shown for trapping and dragging active sperm, holding them in the optical plane as well as performing force measurements for sperm motility. Both techniques are used as a "non-contact" option in artificial fertilization to facilitate sperm-oocyte fusion. The video shows mainly microscopic shots, but also explains some technical procedures by means of animation sequences.
Keywords
in vitro fertilisation
reproduction medicine
fertility / infertility
laser micro beam
tweezers, optical
blastomere fusion
Zona pellucida
ovum
sperm
double beam laser trap
artificial insemination
micromanipulation
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  258 ms - page object

Version

AV-Portal 3.9.1 (0da88e96ae8dbbf323d1005dc12c7aa41dfc5a31)