Preparation of Agarose Pads for Microscopy of C. elegans
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Computer animationProgram flowchartEngineering drawing
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Engineering drawingProgram flowchartBacteriaComputer animation
Transcript: English(auto-generated)
00:01
Preparation of Agarose pads for microscopy of C. elegans. First, pipe at 50 microliters of a 2% Agarose solution on a microscope slide. Then, place a second slide on top and let it stand at room temperature for 5 minutes to harden.
00:28
In the meantime, wash a worm plate with 2 milliliters of M9 buffer to get the worms in suspension.
00:46
Then, pipe at the suspension in an Eppendorf cube and centrifuge for 2 minutes at 5000 g.
01:06
Now, a worm plate is visible at the bottom of the tube. Discard most of the supernatant, but leave a small rest for resuspension.
01:42
Resuspend the worms by flicking against the tube. Now, separate the two Agarose slides and pipe at 10 microliters of worm suspension on the Agarose pad.
02:28
Next, at 10 microliters of a 60 millimolar sodium acid solution to immobilize the worms.
02:46
Finally, place a coverslip on top of the pad. Now, the worms are ready for inspection under the microscope.